GFP-CLCb (EED/QQN)
(Plasmid #47422)

• Depositing Lab: Peter McPherson
• Publication: Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):168-73. doi: 10.1073/pnas.0707269105. Epub 2007 Dec 28.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CLCb EED/QQN
• Alt name: CLCb
• Alt name: Clathrin light chain b
• Species: H. sapiens (human)
• Mutation: EED changed to QQN, in aa20-41 region
• Entrez Gene: CLTB (a.k.a. LCB)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry