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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Tag2B
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 4324
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVac14
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Alt nameTRX
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SpeciesH. sapiens (human)
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Entrez GeneVAC14 (a.k.a. ArPIKfyve, TAX1BP2, TRX)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-Vac 14 Full Length was a gift from Peter McPherson (Addgene plasmid # 47418 ; http://n2t.net/addgene:47418 ; RRID:Addgene_47418) -
For your References section:
Binding of Vac14 to neuronal nitric oxide synthase: Characterisation of a new internal PDZ-recognition motif. Lemaire JF, McPherson PS. FEBS Lett. 2006 Dec 22;580(30):6948-54. Epub 2006 Dec 4. 10.1016/j.febslet.2006.11.061 PubMed 17161399