Intersectin I SH3 D domain (human)
(Plasmid #47416)

• Depositing Lab: Peter McPherson
• Publication: Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7.

Backbone

• Vector backbone: pGEX-4T1
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Intersectin SH3 domain D
• Alt name: Intersectin
• Species: H. sapiens (human)
• Mutation: only SH3 domain D
• Entrez Gene: ITSN1 (a.k.a. ITSN, SH3D1A, SH3P17)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry