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Addgene

Endophilin SH3 domains
(Plasmid #47401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2tk
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Endophilin
  • Insert Size (bp)
    1050
  • Mutation
    SH3 domain (aa 292-352)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Endophilin SH3 domains was a gift from Peter McPherson (Addgene plasmid # 47401 ; http://n2t.net/addgene:47401 ; RRID:Addgene_47401)

  • For your References section:

    Synaptojanin forms two separate complexes in the nerve terminal. Interactions with endophilin and amphiphysin. Micheva KD, Kay BK, McPherson PS. J Biol Chem. 1997 Oct 24;272(43):27239-45. 10.1074/jbc.272.43.27239 PubMed 9341169
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