Intersectin SH3 domain A
(Plasmid #47396)

• Depositing Lab: Peter McPherson
• Publication: Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7.

Backbone

• Vector backbone: pGEX-2t
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Intersectin SH3 domain A
• Alt name: Intersectin
• Mutation: SH3 domain A
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry