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Purpose(Empty Backbone) Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size (bp) 7044
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Vector typeMammalian Expression, TALEN
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- SV40 NLS (N terminal on insert)
- HA (C terminal on insert)
- SV40 NLS (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-GoldenGate-VP64 was a gift from Charles Gersbach (Addgene plasmid # 47389 ; http://n2t.net/addgene:47389 ; RRID:Addgene_47389) -
For your References section:
Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA. Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3. 10.1038/nmeth.2361 PubMed 23377379
Map uploaded by the depositor.
