pHT-6xR.ads-PxylA-DI
(Plasmid #47386)

• Purpose: Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria
• Depositing Lab: Heng-Phon Too
• Publication: Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.

Backbone

• Vector backbone: pHT01
• Backbone manufacturer: Mo Bi Tec
• Backbone size w/o insert (bp): 7956
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ads
• Alt name: amorphadiene synthase
• Species: Artemisia annua
• Insert Size (bp): 1656
• GenBank ID: JQ319661.1
• Promoter: Pgrac
• Tags / Fusion Proteins:
  • 6xR (N terminal on insert)
  • His (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: LacI-R

Gene/Insert 2

• Gene/Insert name: dxs
• Alt name: 1-deoxy-D-xylulose-5-phosphate synthase
• Alt name: DXP-synthase
• Species: B. subtilis
• Insert Size (bp): 1899
• GenBank ID: 938609
• Promoter: PxylA

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA

Gene/Insert 3

• Gene/Insert name: idi
• Alt name: isopentenyl-diphosphate delta-isomerase
• Species: B. subtilis
• Insert Size (bp): 1050
• GenBank ID: 11239955
• Promoter: PxylA

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry