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PurposeRosa 26 targeting vector
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47381 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneROSA26-1
- Backbone size w/o insert (bp) 9846
- Total vector size (bp) 11946
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Vector typeMouse Targeting
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameM2rtTA
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Insert Size (bp)1500
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer M13-pUC-Fwd (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namepgk-puro
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Insert Size (bp)1600
Resource Information
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A portion of this plasmid was derived from a plasmid made byM2-rtTA was obtained from Dr. H. Bujard
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R26P-M2rtTA was a gift from Rudolf Jaenisch (Addgene plasmid # 47381 ; http://n2t.net/addgene:47381 ; RRID:Addgene_47381) -
For your References section:
Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R. Genesis. 2006 Jan . 44(1):23-8. 10.1002/gene.20180 PubMed 16400644