pCMV10/3xflag-Clock L57E/L113E/W284A
(Plasmid
#47343)
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Purposetriple mutation used for co-IP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV103Xflag
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Backbone manufacturersigma
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameClock
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SpeciesM. musculus (mouse)
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MutationL57E/L113E/W284A
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Entrez GeneClock (a.k.a. 5330400M04Rik, KAT13D)
- Promoter CMV
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Tag
/ Fusion Protein
- 3x Flag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV10/3xflag-Clock L57E/L113E/W284A was a gift from Joseph Takahashi (Addgene plasmid # 47343 ; http://n2t.net/addgene:47343 ; RRID:Addgene_47343) -
For your References section:
Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex. Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, Zhang H, Takahashi JS. Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804. Epub 2012 May 31. 10.1126/science.1222804 PubMed 22653727