pfastbacHTb/mClock26-384
(Plasmid
#47336)
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PurposemClock fragment cloned into pfastbacHTb vector with Hexa His tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepfastbacHTb
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameClock
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SpeciesM. musculus (mouse)
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Mutationaa 26-384
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Entrez GeneClock (a.k.a. 5330400M04Rik, KAT13D)
- Promoter PH
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- Tev (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer polyhedrin_fwd_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pfastbacHTb/mClock26-384 was a gift from Joseph Takahashi (Addgene plasmid # 47336 ; http://n2t.net/addgene:47336 ; RRID:Addgene_47336) -
For your References section:
Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex. Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, Zhang H, Takahashi JS. Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804. Epub 2012 May 31. 10.1126/science.1222804 PubMed 22653727