pcDNA5-H2B-AID-EYFP
(Plasmid #47329)

• Purpose: for plasmid integration using the Flp-In system. AID at C-terminal
• Depositing Lab: Don Cleveland
• Publication: Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6875
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: histone H2B
• Alt name: H2B
• Species: H. sapiens (human)
• Insert Size (bp): 6875
• GenBank ID: NP_066402.2
• Entrez Gene: H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
• Tags / Fusion Proteins:
  • AID (C terminal on insert)
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CTTAAGCTTACCGGTGCCGCCACCatgccagagccagcgaagtctgctcc
• 3′ sequencing primer: CAT CCC GGG CTT AGC GCT GGT GTA CTT GGT GAT GGC CTT AGT ACC CTC GG

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The AID ORF was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Nishimura et al., 2009, Nature Methods