pBabe TIR1-9myc
(Plasmid #47328)

• Purpose: For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term NES and C-term 9x myc tag
• Depositing Lab: Don Cleveland
• Publication: Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12.

Backbone

• Vector backbone: pBabe-puro
• Total vector size (bp): 7300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRANSPORT INHIBITOR RESPONSE 1
• Alt name: TIR1
• Species: O. sativa
• Insert Size (bp): 2091
• GenBank ID: NP_001052659.1
• Tags / Fusion Proteins:
  • NES (nuclear export signal - ALQKKLEELELDEGG) (N terminal on insert)
  • 9x Myc (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Nishimura et al., 2009, Nature Methods

Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/