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Addgene

TAL3427
(Plasmid #47146)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JDS70
  • Backbone manufacturer
    Addgene plasmid #32285
  • Backbone size w/o insert (bp) 6447
  • Vector type
    Mammalian Expression ; T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZebrafishCommunity-dyrk1b-right
  • Alt name
    dyrk1b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1430
  • Entrez Gene
    dyrk1b (a.k.a. si:ch211-225p5.1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3X Flag (N terminal on backbone)
    • WT FOKI (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target binding site: TGTTTGTTCATGAGCTCA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL3427 was a gift from Keith Joung (Addgene plasmid # 47146 ; http://n2t.net/addgene:47146 ; RRID:Addgene_47146)

  • For your References section:

    Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. 10.1038/nbt.1934 PubMed 21822241
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