pCR4-*mRFP
(Plasmid
#47118)
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PurposeCan be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3956
- Total vector size (bp) 4655
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name*mRFP
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MutationNo start codon
- Promoter Lac
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR4-*mRFP was a gift from Stephen Ekker (Addgene plasmid # 47118 ; http://n2t.net/addgene:47118 ; RRID:Addgene_47118) -
For your References section:
In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8. 10.1038/nmeth.1606 PubMed 21552255