pcDNA-dCas9-VP64 Citations (20)
Originally described in: RNA-guided gene activation by CRISPR-Cas9-based transcription factors.Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. PubMed Journal
Articles Citing pcDNA-dCas9-VP64
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| Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Kabadi AM, Gersbach CA. Methods. 2014 Sep;69(2):188-97. doi: 10.1016/j.ymeth.2014.06.014. Epub 2014 Jul 8. PubMed |
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
PubMed
Associated Plasmids |
| CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed |
| Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter. Ji H, Jiang Z, Lu P, Ma L, Li C, Pan H, Fu Z, Qu X, Wang P, Deng J, Yang X, Wang J, Zhu H. Mol Ther. 2016 Mar;24(3):508-21. doi: 10.1038/mt.2016.7. Epub 2016 Jan 18. PubMed |
| Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed |
| Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Garcia-Bloj B, Moses C, Sgro A, Plani-Lam J, Arooj M, Duffy C, Thiruvengadam S, Sorolla A, Rashwan R, Mancera RL, Leisewitz A, Swift-Scanlan T, Corvalan AH, Blancafort P. Oncotarget. 2016 Sep 13;7(37):60535-60554. doi: 10.18632/oncotarget.11142. PubMed |
| Targeted Gene Activation Using RNA-Guided Nucleases. Brown A, Woods WS, Perez-Pinera P. Methods Mol Biol. 2017;1468:235-50. doi: 10.1007/978-1-4939-4035-6_16. PubMed |
| Orthogonal genetic regulation in human cells using chemically induced CRISPR/Cas9 activators. Bao Z, Jain S, Jaroenpuntaruk V, Zhao H. ACS Synth Biol. 2017 Jan 5. doi: 10.1021/acssynbio.6b00313. PubMed |
Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2.
Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL.
Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.
PubMed
Associated Plasmids |
| Targeted Reactivation of FMR1 Transcription in Fragile X Syndrome Embryonic Stem Cells. Haenfler JM, Skariah G, Rodriguez CM, Monteiro da Rocha A, Parent JM, Smith GD, Todd PK. Front Mol Neurosci. 2018 Aug 15;11:282. doi: 10.3389/fnmol.2018.00282. eCollection 2018. PubMed |
CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency.
Matharu N, Rattanasopha S, Tamura S, Maliskova L, Wang Y, Bernard A, Hardin A, Eckalbar WL, Vaisse C, Ahituv N.
Science. 2018 Dec 13. pii: science.aau0629. doi: 10.1126/science.aau0629.
PubMed
Associated Plasmids |
| Heat-Triggered Remote Control of CRISPR-dCas9 for Tunable Transcriptional Modulation. Gamboa L, Phung EV, Li H, Meyers JP, Hart AC, Miller IC, Kwong GA. ACS Chem Biol. 2020 Feb 21;15(2):533-542. doi: 10.1021/acschembio.9b01005. Epub 2020 Jan 13. PubMed |
| In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators. Polkoff KM, Chung J, Simpson SG, Gleason K, Piedrahita JA. CRISPR J. 2020 Oct;3(5):409-418. doi: 10.1089/crispr.2020.0037. PubMed |
| TALEN outperforms Cas9 in editing heterochromatin target sites. Jain S, Shukla S, Yang C, Zhang M, Fatma Z, Lingamaneni M, Abesteh S, Lane ST, Xiong X, Wang Y, Schroeder CM, Selvin PR, Zhao H. Nat Commun. 2021 Jan 27;12(1):606. doi: 10.1038/s41467-020-20672-5. PubMed |
| CRISPRa-Mediated Upregulation of scn1laa During Early Development Causes Epileptiform Activity and dCas9-Associated Toxicity. Weuring WJ, Dilevska I, Hoekman J, van de Vondervoort J, Koetsier M, van 't Slot RH, Braun KPJ, Koeleman BPC. CRISPR J. 2021 Aug;4(4):575-582. doi: 10.1089/crispr.2021.0013. PubMed |
Modular Synthetic Biology Toolkit for Filamentous Fungi.
Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM.
ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
PubMed
Associated Plasmids |
| A beta-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer. Rappaport JA, Entezari AA, Caspi A, Caksa S, Jhaveri AV, Stanek TJ, Ertel A, Kupper J, Fortina PM, McMahon SB, Jaynes JB, Snook AE, Waldman SA. Cell Mol Gastroenterol Hepatol. 2022;13(4):1276-1296. doi: 10.1016/j.jcmgh.2021.12.014. Epub 2021 Dec 22. PubMed |
| Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators. Ren C, Li H, Liu Y, Li S, Liang Z. Hortic Res. 2022 Jan 18. pii: 6510199. doi: 10.1093/hr/uhab037. PubMed |
| Robust, Durable Gene Activation In Vivo via mRNA-Encoded Activators. Beyersdorf JP, Bawage S, Iglesias N, Peck HE, Hobbs RA, Wroe JA, Zurla C, Gersbach CA, Santangelo PJ. ACS Nano. 2022 Mar 31. doi: 10.1021/acsnano.1c10631. PubMed |
| Epigenome editing based on CRISPR/dCas9(p300) facilitates transdifferentiation of human fibroblasts into Leydig-like cells. Huang H, Zhang W, Zhang J, Zhao A, Jiang H. Exp Cell Res. 2023 Apr 15;425(2):113551. doi: 10.1016/j.yexcr.2023.113551. Epub 2023 Mar 11. PubMed |
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