pcDNA-dCas9-VP64
(Plasmid #47107)

• Purpose: Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells
• Depositing Lab: Charles Gersbach
• Publication: Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 9812
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-VP64
• Species: S. Pyogenes
• Insert Size (bp): 4446
• Mutation: D10A, H840A (catalytically inactive)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)
  • VP64 (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Duke Limited Ancillary Agreement for dCas9
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/