pcDNA3.1-MAP3K5 (WT)
(Plasmid #47104)

• Purpose: Expresses wild type human MAP3K5 in mammalian cells
• Depositing Lab: Yardena Samuels
• Publication: MAP3K5 expression plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 9597
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAP3K5
• Alt name: ASK1
• Species: H. sapiens (human)
• Insert Size (bp): 4170
• Mutation: NA
• Entrez Gene: MAP3K5 (a.k.a. ASK1, MAPKKK5, MEKK5)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this plasmid proved difficult to sequence and Addgene was unable to confirm the presence of the FLAG tag. Please keep this in mind when ordering.