pPong_v2
(Plasmid #47101)

• Purpose: Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.
• Depositing Lab: Wayne Parrott
• Publication: Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. doi: 10.1104/pp.111.181206. Epub 2011 Aug 15.

Backbone

• Vector backbone: pUQ218
• Backbone size w/o insert (bp): 9900
• Total vector size (bp): 17874
• Modifications to backbone: RNAi hairpin cassette removed.
• Vector type: plant expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Growth instructions: Very large plasmid--may need extra time for growth.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mPing
• Species: Oryza sativa
• Insert Size (bp): 430
• Promoter: no promoter

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: Pong Open Reading Frame 1 (ORF1)
• Alt name: Pong
• Species: O. Sativa
• Promoter: GmUbi_XS

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 3

• Gene/Insert name: Pong Transposase
• Alt name: Tpase
• Species: Oryza sativa
• Insert Size (bp): 1449
• Promoter: GmUbi_AP

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

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