pPing
(Plasmid #47100)

• Purpose: Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.
• Depositing Lab: Wayne Parrott
• Publication: Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. doi: 10.1104/pp.111.181206. Epub 2011 Aug 15.

Backbone

• Vector backbone: pUHN4
• Backbone manufacturer: Joe Nairn, University of Georgia
• Backbone size w/o insert (bp): 5561
• Total vector size (bp): 11686
• Vector type: Yeast Expression ; plant expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Ping cDNA
• Alt name: ORF1 and Tpase
• Species: Oryza sativa
• Insert Size (bp): 3565
• Promoter: CaMV35s

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: mPing
• Species: Oryza sativa
• Insert Size (bp): 430
• Promoter: CaMV35s
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 3

• Gene/Insert name: hygromycin resistance gene
• Alt name: hph
• Species: E. coli
• Insert Size (bp): 1026
• Promoter: StUbi3

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry