pET12a-AL-103 Y91F
(Plasmid
#47093)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET12a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4674
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAL-103 Y91F
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Alt nameimmunoglobulin kappa light chain variable region
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Alt nameamyloidosis protein
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SpeciesH. sapiens (human)
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MutationY91F
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Entrez GeneIGKV3-20 (a.k.a. 13K18, A27, IGKV320)
- Promoter T7
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Tag
/ Fusion Protein
- ompT (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET12a-AL-103 Y91F was a gift from Marina Ramirez-Alvarado (Addgene plasmid # 47093 ; http://n2t.net/addgene:47093 ; RRID:Addgene_47093) -
For your References section:
Tyrosine residues mediate fibril formation in a dynamic light chain dimer interface. DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, Ramirez-Alvarado M. J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 10.1074/jbc.M112.362921. Epub 2012 Jun 27. 10.1074/jbc.M112.362921 PubMed 22740699