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pET12a-AL-103 Y32F Y96F
(Plasmid #47092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET12a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4674
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AL-103 Y32F Y96F
  • Alt name
    immunoglobulin kappa light chain variable region
  • Alt name
    amyloidosis protein
  • Species
    H. sapiens (human)
  • Mutation
    Y32F Y96F
  • Entrez Gene
    IGKV3-20 (a.k.a. 13K18, A27, IGKV320)
  • Promoter T7
  • Tag / Fusion Protein
    • ompT (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET12a-AL-103 Y32F Y96F was a gift from Marina Ramirez-Alvarado (Addgene plasmid # 47092 ; http://n2t.net/addgene:47092 ; RRID:Addgene_47092)

  • For your References section:

    Tyrosine residues mediate fibril formation in a dynamic light chain dimer interface. DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, Ramirez-Alvarado M. J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 10.1074/jbc.M112.362921. Epub 2012 Jun 27. 10.1074/jbc.M112.362921 PubMed 22740699
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