pET12a-AL-09 FL H87Y C214S
(Plasmid
#47087)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET12a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4674
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAL-09 FL H87Y C214S
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Alt nameimmunoglobulin kappa light chain variable region
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Alt nameamyloidosis protein
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SpeciesH. sapiens (human)
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MutationH87Y C214S
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Entrez GeneIGKV3-20 (a.k.a. 13K18, A27, IGKV320)
- Promoter T7
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Tag
/ Fusion Protein
- ompT (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET12a-AL-09 FL H87Y C214S was a gift from Marina Ramirez-Alvarado (Addgene plasmid # 47087 ; http://n2t.net/addgene:47087 ; RRID:Addgene_47087) -
For your References section:
Role of mutations in the cellular internalization of amyloidogenic light chains into cardiomyocytes. Levinson RT, Olatoye OO, Randles EG, Howell KG, DiCostanzo AC, Ramirez-Alvarado M. Sci Rep. 2013;3:1278. doi: 10.1038/srep01278. 10.1038/srep01278 PubMed 23417147