pET12a-AL-09 FL
(Plasmid
#47081)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET12a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4674
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAL-09
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Alt nameimmunoglobulin kappa light chain variable region
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Alt nameamyloidosis protein
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SpeciesH. sapiens (human)
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Entrez GeneIGKV3-20 (a.k.a. 13K18, A27, IGKV320)
- Promoter T7
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Tag
/ Fusion Protein
- ompT (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET12a-AL-09 FL was a gift from Marina Ramirez-Alvarado (Addgene plasmid # 47081 ; http://n2t.net/addgene:47081 ; RRID:Addgene_47081) -
For your References section:
The effects of sodium sulfate, glycosaminoglycans, and Congo red on the structure, stability, and amyloid formation of an immunoglobulin light-chain protein. McLaughlin RW, De Stigter JK, Sikkink LA, Baden EM, Ramirez-Alvarado M. Protein Sci. 2006 Jul;15(7):1710-22. Epub 2006 Jun 2. 10.1110/ps.051997606 PubMed 16751605
