pBMN YFP-ltDHFR(DD)
(Plasmid #47080)

• Purpose: ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
• Depositing Lab: Thomas Wandless
• Publication: Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393.

Backbone

• Vector backbone: pBMN
• Backbone manufacturer: Garry Nolan
• Backbone size w/o insert (bp): 6943
• Vector type: Retroviral
• Selectable markers: HcRed Fluorescence

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP-ltDHFR(DD)
• Alt name: folA dihydrofolate reductase ( destable domain) with YFP fusion for low temperature
• Species: E. coli
• Insert Size (bp): 1233
• Mutation: amino acids mutations: R12H, N37D, N59D, G67S, D132G
• GenBank ID: AAC73159
• Promoter: LTR
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XnoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: lab32
• 3′ sequencing primer: LC47

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This C-terminal ltDHFR(DD) plasmid is the clone#7 in Table 1