pJBEI-6410
(Plasmid #47049)

• Purpose: BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
• Depositing Lab: Taek Soon Lee
• Publication: Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.

Backbone

• Vector backbone: pBbA5a
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 15146
• Vector type: Bacterial Expression, Synthetic Biology ; BglBrick

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
• Species: S. cerevisiae (budding yeast); Mentha spicata, Abies grandis and Staphylococcus aureus
• Insert Size (bp): 11500
• Promoter: PlacUV5

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, BglII (not destroyed)
• 3′ cloning site: BamHI, XhoI (not destroyed)
• 5′ sequencing primer: F1
• 3′ sequencing primer: R1

Resource Information

• Supplemental Documents:
  • Plasmid map
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry