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Addgene

Beclin-HA S-D
(Plasmid #46995)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pqcxih
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    becn1
  • Species
    M. musculus (mouse)
  • Mutation
    S14D
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beclin-HA S-D was a gift from Kunliang Guan (Addgene plasmid # 46995 ; http://n2t.net/addgene:46995 ; RRID:Addgene_46995)

  • For your References section:

    ULK1 induces autophagy by phosphorylating Beclin-1 and activating VPS34 lipid kinase. Russell RC, Tian Y, Yuan H, Park HW, Chang YY, Kim J, Kim H, Neufeld TP, Dillin A, Guan KL. Nat Cell Biol. 2013 Jul;15(7):741-50. doi: 10.1038/ncb2757. Epub 2013 May 19. 10.1038/ncb2757 PubMed 23685627
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