pEGFP-C1-FLAG-XRCC4
(Plasmid #46959)

• Purpose: Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.
• Depositing Lab: Steve Jackson
• Publication: Britton et al J Cell Biol. 2013 Jul 29.

Backbone

• Vector backbone: pEGFP-C1-FLAG
• Backbone size w/o insert (bp): 4766
• Total vector size (bp): 5785
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XRCC4
• Species: H. sapiens (human)
• Insert Size (bp): 1005
• GenBank ID: NM_022406.2
• Entrez Gene: XRCC4 (a.k.a. SSMED, hXRCC4)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP-2xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CATGGTCCTGCTGGAGTTCGTG
• 3′ sequencing primer: GCAAGTAAAACCTCTACAAATGTGG

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry