pAAV-Ku70-TC
(Plasmid #46955)

• Purpose: Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.
• Depositing Lab: Steve Jackson
• Publication: Britton et al J Cell Biol. 2013 Jul 29.

Backbone

• Vector backbone: pAAV-MCS2
• Backbone size w/o insert (bp): 4650
• Total vector size (bp): 7319
• Vector type: AAV ; r

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XRCC6 targeting construct
• Alt name: Ku70
• Species: H. sapiens (human)
• Insert Size (bp): 4426
• GenBank ID: NT_011520
• Entrez Gene: XRCC6 (a.k.a. CTC75, CTCBF, G22P1, KU70, ML8, TLAA)
• Tag / Fusion Protein:
  • GFP-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI/Bsp12OI (destroyed during cloning)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry