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PurposeGFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCIneo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 6220
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
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SpeciesAequorea victoria
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIneoEGFP was a gift from John Schiller (Addgene plasmid # 46949 ; http://n2t.net/addgene:46949 ; RRID:Addgene_46949) -
For your References section:
Production of papillomavirus-based gene transfer vectors. Buck CB, Thompson CD. Curr Protoc Cell Biol. 2007 Dec;Chapter 26:Unit 26.1. doi: 10.1002/0471143030.cb2601s37. 10.1002/0471143030.cb2601s37 PubMed 18228512
Map uploaded by the depositor.
