pLXIN-Ap1g1-FKBP
(Plasmid
#46946)
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PurposeRetroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46946 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLXIN
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAp1g1-FKBP
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SpeciesH. sapiens (human), M. musculus (mouse)
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Tag
/ Fusion Protein
- Mainly mouse Ap1g1 cDNA, with a fragment of brain-specific splice variant of Ap2a1, an FKBP domain, and siRNA-resistance
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLXIN-Ap1g1-FKBP was a gift from Margaret Robinson (Addgene plasmid # 46946 ; http://n2t.net/addgene:46946 ; RRID:Addgene_46946) -
For your References section:
Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria. Robinson MS, Sahlender DA, Foster SD. Dev Cell. 2010 Feb 16;18(2):324-31. doi: 10.1016/j.devcel.2009.12.015. 10.1016/j.devcel.2009.12.015 PubMed 20159602