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Addgene

pEYFP-Mitotrap
(Plasmid #46942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mitotrap
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast), Synthetic; Aequorea victoria
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Tag / Fusion Protein
    • This is a fusion between a yeast mitochondrial targeting signal, a YFP reporter, and the FRB rapamycin-binding domain

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-Mitotrap was a gift from Margaret Robinson (Addgene plasmid # 46942 ; http://n2t.net/addgene:46942 ; RRID:Addgene_46942)

  • For your References section:

    Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria. Robinson MS, Sahlender DA, Foster SD. Dev Cell. 2010 Feb 16;18(2):324-31. doi: 10.1016/j.devcel.2009.12.015. 10.1016/j.devcel.2009.12.015 PubMed 20159602
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