pCMV4a-hFJX1-Flag
(Plasmid
#46931)
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Purposeexpression of human FJX1-FLAG in mammalian cells
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag 4A
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4300
- Total vector size (bp) 5800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFJX1
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Alt namefour jointed box 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Entrez GeneFJX1
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV4a-hFJX1-Flag was a gift from R.D. Beauchamp & Natasha Deane (Addgene plasmid # 46931 ; http://n2t.net/addgene:46931 ; RRID:Addgene_46931)