pcDNA-zeo-hFJX1-myc
(Plasmid
#46930)
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Purposeexpression of human FJX1-myc in mammalian cells
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46930 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Zeo(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5015
- Total vector size (bp) 6500
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFJX1
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Alt namefour jointed box 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Entrez GeneFJX1
- Promoter CMV
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-zeo-hFJX1-myc was a gift from R.D. Beauchamp & Natasha Deane (Addgene plasmid # 46930 ; http://n2t.net/addgene:46930 ; RRID:Addgene_46930)