pTDH3-dCas9
(Plasmid #46920)

• Purpose: Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter
• Depositing Labs: Stanley Qi, Jonathan Weissman
• Publication: Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.

Backbone

• Vector backbone: pJED103
• Backbone size w/o insert (bp): 6900
• Total vector size (bp): 11054
• Vector type: Yeast Expression, CRISPR
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Insert Size (bp): 4135
• Promoter: TDH3

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: gGAGCTCactcgGAATTCagtctACTAGT

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Cas9 contains a N612K mutation that does not effect the function of the enzyme.

For more information on Qi and Wiessman Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Qi-weissman/ and http://www.addgene.org/crispr/qi/