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pRK5-Myc-SH3BP4 W92A
(Plasmid #46892)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-myc
  • Backbone manufacturer
    clonetch
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SH3BP4 W92A
  • Alt name
    BOG25
  • Alt name
    SH3-domain binding protein 4
  • Alt name
    SH3BP4
  • Species
    H. sapiens (human)
  • Mutation
    W92A (mutated SH3 domain)
  • Entrez Gene
    SH3BP4 (a.k.a. BOG25, TTP)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutagenesis was performed using a site-directed mutagenesis kit (Stratagene) and the following primer:
cacatctggcggtgaggcgtggtacgcacacaac

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-Myc-SH3BP4 W92A was a gift from Do-Hyung Kim (Addgene plasmid # 46892 ; http://n2t.net/addgene:46892 ; RRID:Addgene_46892)

  • For your References section:

    SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 signaling. Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH. Mol Cell. 2012 Jun 29;46(6):833-46. doi: 10.1016/j.molcel.2012.04.007. Epub 2012 May 9. 10.1016/j.molcel.2012.04.007 PubMed 22575674
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