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Addgene

pDL276
(Plasmid #46881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46881 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDL276
  • Backbone size (bp) 6984
  • Modifications to backbone
    See associated article for detailed construction information
  • Vector type
    Bacterial Expression ; E. coli/Gram-positive Bacterial Shuttle Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For E. coli, grow in Luria broth (LB) agar containing 50μg/mL kanamycin. For E. faecalis, select at 500μg/mL in culture and 1000μg/mL on plates.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13_pUC_rev
  • 3′ sequencing primer M13_pUC_fwd; pBAD_rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL276 was a gift from Gary Dunny (Addgene plasmid # 46881)

  • For your References section:

    Improved electroporation and cloning vector system for gram-positive bacteria. Dunny GM, Lee LN, LeBlanc DJ. Appl Environ Microbiol. 1991 Apr;57(4):1194-201. PubMed 1905518
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