Skip to main content
Addgene

pMA3411
(Plasmid #46877)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46877 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pMA1662
  • Backbone size w/o insert (bp) 5684
  • Total vector size (bp) 7567
  • Vector type
    Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    soluble guanylate cyclase1 beta 3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1920
  • Mutation
    E473K, C541D
  • Entrez Gene
    Gucy1b3
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer agccctttgtacaccctaag
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA was purchased from Open Biosystems (Huntsville, AL; catalogue No. 7104600)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA3411 was a gift from Mikhail Alexeyev & Troy Stevens (Addgene plasmid # 46877 ; http://n2t.net/addgene:46877 ; RRID:Addgene_46877)

  • For your References section:

    Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability. Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T. J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 10.1074/jbc.M111.301440. Epub 2012 May 25. 10.1074/jbc.M111.301440 PubMed 22637478
Related items: