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Addgene

pMA3383
(Plasmid #46875)

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Item Catalog # Description Quantity Price (USD)
Plasmid 46875 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMA1662
  • Backbone manufacturer
    Homemade
  • Backbone size w/o insert (bp) 5684
  • Total vector size (bp) 7527
  • Vector type
    Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    soluble guanylate cyclase 1beta 3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1880
  • Entrez Gene
    Gucy1b3
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer agccctttgtacaccctaag
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA was purchased from Open Biosystems (Huntsville, AL; catalogue No. 7104600)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA3383 was a gift from Mikhail Alexeyev & Troy Stevens (Addgene plasmid # 46875 ; http://n2t.net/addgene:46875 ; RRID:Addgene_46875)

  • For your References section:

    Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that increases endothelial tau phosphorylation and permeability. Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T. J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 10.1074/jbc.M111.301440. Epub 2012 May 25. 10.1074/jbc.M111.301440 PubMed 22637478
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