pGL3-24p3/LCN2-282C/EBP mut-LUC
(Plasmid #46868)

• Purpose: murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter
• Depositing Lab: Sarah Gaffen
• Publication: Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5100
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 24p3/lipocalin 2 proximal promoter
• Alt name: LCN2 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 282
• Mutation: CEBP site is mutated (see comments)
• Promoter: 24p3 promoter (inactive)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu I (not destroyed)
• 3′ cloning site: Xho I (destroyed during cloning)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Primers used to create the C/EBP mutation were (lower case are mutations):
5-CAG CCC TTC CTG gca CTt ggC CTT GCA CAG TTC CGA C-3 and
5-AAC TGT GCA AGG cca AGt gcC AGG AAG GGC TGC AGG G-3