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pGL3-24p3/LCN2-145-LUC
(Plasmid #46866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 4963
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    24p3/lipocalin 2 proximal promoter
  • Alt name
    LCN2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    145
  • Promoter 24p3 promoter (inactive)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (destroyed during cloning)
  • 3′ cloning site Xho I (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-24p3/LCN2-145-LUC was a gift from Sarah Gaffen (Addgene plasmid # 46866 ; http://n2t.net/addgene:46866 ; RRID:Addgene_46866)

  • For your References section:

    Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598
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