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Addgene

pCMV4-Flag-mIL-17RA/CFP.Hygro
(Plasmid #46861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4-Hygro
  • Backbone size w/o insert (bp) 6473
  • Total vector size (bp) 10450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL-17RA-CFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • Promoter CMV
  • Tags / Fusion Proteins
    • fused to CFP (C terminal on insert)
    • Flag/IL-3 signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Cla (unknown if destroyed)
  • 5′ sequencing primer GAACCGTCAGAATTG
  • 3′ sequencing primer CTTCCAAGGCCAGGAGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV4-Flag-mIL-17RA/CFP.Hygro was a gift from Sarah Gaffen (Addgene plasmid # 46861 ; http://n2t.net/addgene:46861 ; RRID:Addgene_46861)

  • For your References section:

    Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598
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