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pX21
(Plasmid #46849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX1
  • Backbone size w/o insert (bp) 2977
  • Total vector size (bp) 15806
  • Modifications to backbone
    Multi-step assembling as large construct
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For culture in LB use Carbenicillin instead of Ampicillin
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    129/Ola mouse Trp53 gene, Puro and attP sites
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    12830
  • Mutation
    attP flanked PGK-Neo

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX21 was a gift from Monica Hollstein (Addgene plasmid # 46849 ; http://n2t.net/addgene:46849 ; RRID:Addgene_46849)

  • For your References section:

    Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform. Wei QX, Odell AF, van der Hoeven F, Hollstein M. Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15. 10.4161/cc.10.8.15303 PubMed 21445009
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