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psd44-iGFP-MFGE8-long
(Plasmid #46847)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psd44iGFP
  • Backbone size w/o insert (bp) 10500
  • Modifications to backbone
    IRES2 and EGFP was added to psd44 puro to make psd44-iGFP puro.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MFGE8
  • Alt name
    milk fat globule-EGF factor 8 protein
  • Alt name
    SED1
  • Alt name
    lactadherin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_008594
  • Entrez Gene
    Mfge8 (a.k.a. MFG-E8, MP47, Mfgm, P47, SED1, lactadherin)
  • Promoter PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer hPGK-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derived from ImaGenes NM_008594_pcDNA3.1 (+). See http://www.lifesciences.sourcebioscience.com/genomecube.aspx?kw=MFGE8 for more info.
IRES2 and EGFP was added to psd44 puro to make psd44-iGFP puro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psd44-iGFP-MFGE8-long was a gift from Agnese Mariotti (Addgene plasmid # 46847 ; http://n2t.net/addgene:46847 ; RRID:Addgene_46847)

  • For your References section:

    MFG-E8/lactadherin regulates cyclins D1/D3 expression and enhances the tumorigenic potential of mammary epithelial cells. Carrascosa C, Obula RG, Missiaglia E, Lehr HA, Delorenzi M, Frattini M, Ruegg C, Mariotti A. Oncogene. 2012 Mar 22;31(12):1521-32. doi: 10.1038/onc.2011.356. Epub 2011 Aug 15. 10.1038/onc.2011.356 PubMed 21841820
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