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Addgene

pIVEX-SNAP-GFP
(Plasmid #46846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVEX
  • Backbone manufacturer
    Roche Applied Science
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4737
  • Vector type
    In vitro expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SNAP-GFP fusion
  • Alt name
    SNAP-tag
  • Alt name
    AGT
  • Alt name
    GFP
  • Insert Size (bp)
    1269

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7t
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVEX-SNAP-GFP was a gift from Florian Hollfelder (Addgene plasmid # 46846 ; http://n2t.net/addgene:46846 ; RRID:Addgene_46846)

  • For your References section:

    In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Diamante L, Gatti-Lafranconi P, Schaerli Y, Hollfelder F. Protein Eng Des Sel. 2013 Oct;26(10):713-24. doi: 10.1093/protein/gzt039. Epub 2013 Aug 26. 10.1093/protein/gzt039 PubMed 23980186
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