pIVEX-anchor
(Plasmid
#46844)
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Purpose(Empty Backbone) Template for the amplification of spiking anchors in BeSD
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIVEX
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Backbone manufacturerRoche Applied Science
- Backbone size (bp) 4000
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Modifications to backboneBglII/BamHI digested and self-ligated
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Vector typeIn vitro expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Cloning Information
- 3′ sequencing primer T7t (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVEX-anchor was a gift from Florian Hollfelder (Addgene plasmid # 46844 ; http://n2t.net/addgene:46844 ; RRID:Addgene_46844) -
For your References section:
In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Diamante L, Gatti-Lafranconi P, Schaerli Y, Hollfelder F. Protein Eng Des Sel. 2013 Oct;26(10):713-24. doi: 10.1093/protein/gzt039. Epub 2013 Aug 26. 10.1093/protein/gzt039 PubMed 23980186
Map uploaded by the depositor.
