pCRII-TOPO CMV-cGFP-mMEN b_3' Sense
(Plasmid
#46840)
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PurposeExpress cGFP transcript ending in the wildtype MEN beta triple helix
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 7006
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCoral Green Fluorescent Protein (cGFP)
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Insert Size (bp)720
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII-TOPO CMV-cGFP-mMEN b_3' Sense was a gift from Phil Sharp (Addgene plasmid # 46840 ; http://n2t.net/addgene:46840 ; RRID:Addgene_46840) -
For your References section:
A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails. Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA. Genes Dev. 2012 Nov 1;26(21):2392-407. doi: 10.1101/gad.204438.112. Epub 2012 Oct 16. 10.1101/gad.204438.112 PubMed 23073843