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Addgene

psd44-Lactadherin46
(Plasmid #46830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psd44
  • Backbone manufacturer
    Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
  • Backbone size w/o insert (bp) 9195
  • Total vector size (bp) 10370
  • Modifications to backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MFGE8
  • Alt name
    milk fat globule-EGF factor 8 protein
  • Alt name
    lactadherin
  • Alt name
    BA46
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1196
  • GenBank ID
    NM_005928
  • Entrez Gene
    MFGE8 (a.k.a. BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47)
  • Promoter PGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer hPGK-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Originally from RSPD, Germany, plasmid IOH46424, in pDEST26 as backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psd44-Lactadherin46 was a gift from Agnese Mariotti (Addgene plasmid # 46830 ; http://n2t.net/addgene:46830 ; RRID:Addgene_46830)
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