psd44-G13GA
(Plasmid #46827)

• Purpose: expression of mutant G protein alpha 13 in mammalian cells
• Depositing Lab: Agnese Mariotti
• Publication: Mariotti lab plasmids (unpublished)

Backbone

• Vector backbone: psd44
• Backbone manufacturer: Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
• Backbone size w/o insert (bp): 9195
• Total vector size (bp): 10370
• Modifications to backbone: pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GNA13
• Alt name: guanine nucleotide binding protein (G protein), alpha 13
• Alt name: G protein alpha 13
• Alt name: G alpha 13
• Species: H. sapiens (human)
• Insert Size (bp): 1175
• Mutation: G225A
• Entrez Gene: GNA13 (a.k.a. G13, HG1N)
• Promoter: PGK promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: hPGK-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA1300000) and subsequently the point mutation was added by the Mariotti lab.