psd44-GqQL
(Plasmid #46826)

• Purpose: 2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
• Depositing Lab: Agnese Mariotti
• Publication: Mariotti lab plasmids (unpublished)

Backbone

• Vector backbone: psd44
• Backbone manufacturer: Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
• Modifications to backbone: pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: g protein alpha q
• Alt name: GNAQ
• Alt name: guanine nucleotide binding protein (G protein), q polypeptide
• Species: H. sapiens (human)
• Insert Size (bp): 1120
• Mutation: Q209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)
• Entrez Gene: GNAQ (a.k.a. CMAL, CMC1, G-ALPHA-q, GAQ, SWS)
• Promoter: PGK promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: hPGK-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA0Q000C0).

Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.