psd44-G12QL
(Plasmid #46825)

• Purpose: expression of mutant G protein alpha 12 in mammalian cells
• Depositing Lab: Agnese Mariotti
• Publication: Mariotti lab plasmids (unpublished)

Backbone

• Vector backbone: psd44
• Backbone manufacturer: Dr. Richard Iggo, University of St Andrews, Edinburgh, UK
• Backbone size w/o insert (bp): 9195
• Total vector size (bp): 10380
• Modifications to backbone: pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GNA12
• Alt name: RMP
• Alt name: guanine nucleotide binding protein (G protein) alpha 12
• Species: H. sapiens (human)
• Insert Size (bp): 1185
• Mutation: Q231L (the mutation reduces GTPase activity resulting in a constitutively active phenotype)
• Entrez Gene: GNA12 (a.k.a. HG1M1, NNX3, RMP, gep)
• Promoter: PGK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: hPGK-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA12000C0).